Glycomyces tritici sp. nov., isolated from rhizosphere soil of wheat ( Triticum aestivum L.) and emended description of the genus Glycomyces

A novel actinomycete strain, designated NEAU-C2 T , was isolated from rhizosphere soil of wheat ( Triticum aestivum L.), and subjected to a polyphasic taxonomic study. Morphological and chemotaxonomic properties of this strain were consistent with the description of the genus Glycomyces . Growth was found to occur at a temperature range of 15–40 °C, pH 6–10 and NaCl concentrations of 0–4%. The cell wall was found to contain meso -diaminopimelic acid and the whole cell sugars were identified as galactose, xylose and ribose. The predominant menaquinones were identified as MK-10(H 6 ) and MK-10(H 2 ). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids and an unidentified glycolipid. The major fatty acids were identified as anteiso-C 15:0 , iso-C 16:0 , anteiso-C 17:0 and iso-C 15:0 . 16S rRNA gene sequence similarity studies showed that strain NEAU-C2 T belongs to the genus Glycomyces with high sequence similarity to Glycomyces algeriensis NRRL B-16327 T (99.0% sequence similarity). Some physiological and biochemical properties and low DNA–DNA relatedness values enabled the strain to be differentiated from closely related species of the genus Glycomyces . It is concluded that the isolate can be classified as representing a novel species of the genus Glycomyces , for which the name Glycomyces tritici is proposed. The type strain is NEAU-C2 T (= DSM 104644 T = CGMCC 4.7410 T ).