Integrated control of tobacco black shank by combined use of riboflavin and Bacillus subtilis strain Tpb55

We investigated the effect of riboflavin on the biocontrol activity of Bacillus subtilis Tpb55 against Phytophthora nicotianae ( Pn ), which causes tobacco black shank. Riboflavin (0.2 mg ml −1 ) significantly improved the biocontrol activity of Tpb55 (2.0 × 10 8 cfu ml −1 ). Riboflavin (0.02–0.5 mg ml −1 ) alone could not significantly inhibit Pn growth. However, it enhanced the B. subtilis population, both in vitro and in tobacco roots and significantly increased the activity of defense enzymes, peroxidase, catalase, superoxide dismutase, and β-1,3-glucanase, in the roots of B. subtilis -treated tobacco seedlings. Our results indicate that riboflavin can stimulate the growth of B. subtilis Tpb55 and induce resistance to Pn in tobacco plants. These findings should boost the prospects for practical application of B. subtilis Tpb55 as a biocontrol agent against black shank of tobacco.