Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells.
HYPOXIA: METHODS AND PROTOCOLS（2018）
Abstract
The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.
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Key words
Acute oxygen sensing, Hypoxia, Glomus cells, Enzymatic cell dispersion, O-2-sensitive ion channels, Ionic currents, Patch clamp, Microfluorimetry, Cytosolic calcium
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