Lysobacter tongrenensis sp. nov., isolated from soil of a manganese factory

A Gram-staining negative, aerobic, non-motile, rod-shaped bacterial strain, designated YS-37 T , was isolated from soil in a manganese factory, People’s Republic of China. Based on16S rRNA gene sequence analysis, strain YS-37 T was most closely related to Lysobacter pocheonensis Gsoil 193 T (97.0%), Lysobacter dokdonensis DS-58 T (96.0%) and Lysobacter daecheongensis Dae08 T (95.8%) and grouped together with L. pocheonensis Gsoil 193 T and Lysobacter dokdonensis DS-58 T . The DNA–DNA hybridization value between strain YS-37 T and L. pocheonensis KCTC 12624 T was 43.3% (± 1). The major respiratory quinone of strain YS-37 T was ubiquinone-8, and the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, phosphatidylmethylethaolamine and two unknown lipids. Its major cellular fatty acids (> 5%) were iso-C 15:0 , iso-C 17:1 ω 9c, iso-C 16:0 , iso-C 11:0 3-OH and iso-C 11:0 and the G + C content of the genomic DNA was 67.1 mol%. Strain YS-37 T also showed some biophysical and biochemical differences with the related strains, especially in hydrolysis of casein. The results demonstrated that strain YS-37 T belongs to genus Lysobacter and represents a novel Lysobacter species for which the name Lysobacter tongrenensis sp. nov. is proposed. The type strain is YS-37 T (= CCTCC AB 2016052 T = KCTC 52206 T ).