Site-Specific One-To-One Click Coupling Of Single Proteins To Individual Carbon Nanotubes: A Single-Molecule Approach
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY(2017)
摘要
We report the site-specific coupling of single proteins to individual carbon nanotubes (CNTs) in solution and with single-molecule control. Using an orthogonal Click reaction, Green Fluorescent Protein (GFP) was engineered to contain a genetically encoded azide group and then bound to CNT ends in different configurations: in close proximity or at longer distances from the GFP's functional center. Atomic force microscopy and fluorescence analysis in solution and on surfaces at the single-protein level confirmed the importance of bioengineering optimal protein attachment sites to achieve direct protein-nanotube communication and bridging.
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关键词
Protein-Protein Interactions,Single-Molecule Studies,Nanotubes
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