Saccharopolyspora Aidingensis Sp Nov., An Actinomycete Isolated From A Salt Lake

A novel halophilic actinomycete strain, designated TRM 46074(inverted perpendicular), was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074(inverted perpendicular) was aerobic, Gram-stain-positive and grew optimally with 12% (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain mesodiaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H-4), MK-10(H-8) and MK-10(H-4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C-17 : 0, iso-C-15 : 0, iso-C-16 : 0 and iso-C-17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074(inverted perpendicular) belongs to the genus Saccharopolyspora. Strain TRM 46074(inverted perpendicular) had 16S rRNA gene sequence similarity of 99.6% with the closest described species, Saccharopolyspora ghardaiensis H53(inverted perpendicular). DNA-DNA hybridization between strain TRM 46074(inverted perpendicular) and Saccharopolyspora ghardaiensis H53(inverted perpendicular) showed 58.6% relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074(inverted perpendicular) is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074(inverted perpendicular) (= CCTCC AA 2012014(inverted perpendicular) = JCM 30185(inverted perpendicular)).