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Use of chromatographic and electrophoretic tools for assaying elastase, collagenase, hyaluronidase, and tyrosinase activity.

Syntia Fayad,Philippe Morin,Reine Nehmé

Journal of Chromatography A（2017）

Cited 26|Views5
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Abstract
•Elastase, collagenase, hyaluronidase and tyrosinase assayed by analytical tools.•Various HPLC and CE modes can be used for monitoring enzyme kinetics.•Microscale Thermophoresis is a promising tool for enzyme-effector affinity analysis.•Thin layer chromatography is efficiently used for screening natural enzyme effectors.
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Key words
BGE,BTH,CS,ECM,EMMA,FAC,HA,HNE,Hyal,IB,LIF,MMP,MST,PMMA,SFE,TDLFP,Tyr
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