Pro-inflammatory mediator responses from neonatal airway epithelial cells and early childhood wheeze.

BackgroundAirway epithelial cell (AEC) function differs between children with and without asthma. Here, we associated neonatal AEC function with asthma symptoms at 4 years of age. MethodsNasal AEC were collected from neonates within 48h of birth. Cells were cultured and stimulated with tumor necrosis factor alpha/interleukin-1 beta (TNF/IL-1), lipopolysaccharide (LPS), or house dust mite (HDM). Absolute concentrations of pro-inflammatory mediators in the culture supernatant were quantified and expressed as median [interquartile range] in pg/mg protein. A parent-completed respiratory questionnaire was returned when the child was 4 years old. ResultsAEC were successfully cultured in 139 neonates, of whom 120 were contacted at 4 years and 91 (76%) questionnaires were returned. Sixteen children had wheezed ever and 11 had recent wheeze. At birth, when compared to those with no recent wheeze, supernatants from cultured neonatal AEC from the children with recent wheeze had reduced median IL-8 (CXCL8) release after treatment with culture medium alone (P=0.049), with TNF/IL-1 (P<0.001) and LPS (P=0.004). Additionally, and when compared to those with no recent wheeze, 4 year olds with recent wheeze had reduced neonatal AEC release of IL-6 (P=0.013), GMCSF (P=0.012), and ICAM-1 (P=0.017) after treatment with TNF/IL-1 and reduced release of ICAM-1 (P=0.038) and RANTES (P=0.042) after treatment with HDM. ConclusionsAbnormalities in AEC function are present at birth before the onset of childhood wheeze. The relationship between reduced AEC function at birth and wheeze at 4 years was not exclusive, suggesting that post-natal factors are required for the AEC abnormality to translate into symptoms.