Generation of recombinant affinity reagents against a two-phosphosite epitope of ATF2.

New Biotechnology（2018）

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摘要

•The FHA1 domain can be evolved to recognize a dual-phosphorylated target.•Isolated FHA domain variants are phospho-state specific to an ATF2 epitope.•A new FHA recognition mechanism is utilized by domain variants for interaction with the ATF2 dual-phosphosite epitope.

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关键词

ATF2,ELISA,FHAdomain,PCR,pS,pS/T,pT,S,T

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