RNA Immunoprecipitation Protocol to Identify Protein-RNA Interactions in Arabidopsis thaliana.
Methods in molecular biology (Clifton, N.J.)(2018)
Abstract
The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA-protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.
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Key words
Quantitative PCR,RIP,RNA immunoprecipitation,RNA–protein interaction,Reverse transcription,lncRNA
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