多重位点特异性PCR鉴别人参、三七、西洋参掺杂
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica(2017)
Abstract
为建立一种能同时鉴别人参、三七、西洋参及其掺杂品的方法.通过分析人参属ITS,18S和matK序列,寻找特异性SNP位点设计引物,建立多重位点特异性PCR法,并对不同来源的人参、三七、西洋参样品进行扩增,根据特异性条带大小进行鉴别.在退火温度为60℃,循环数为35时,人参、三七和西洋参分别出现约250,500,1000 bp的特异性条带.该方法对于人参、三七、西洋参相互掺杂的混合样品,人参中掺杂三七或西洋参以及三七中掺杂西洋参或人参的检出限均为0.5%,对西洋参中掺杂人参的检出限为0.5%,掺杂三七的检出限为1%.表明建立的多重位点特异性PCR可用于人参、西洋参、三七的掺杂鉴定.
MoreTranslated text
Key words
Panax ginseng,Panax notoginseng,Panax quinquefolius,admixture,molecular identification,multiplex allele-specific polymerase chain reaction
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined