基于“伴随标志物”在线控制技术的绞股蓝总皂苷纯化工艺及成分鉴定研究

Dong-dong FAN,Yan-hui KUANG,Li-hua DONG,Xiao YE,Liang-mian CHEN,Dong ZHANG,Zhen-shan MA,Jin-yu WANG,Jing-jing ZHU,Zhi-min WANG,De-qin WANG,Chu-yuan LI

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica（2017）

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Abstract

基于“伴随标志物”紫外在线检测技术以绞股蓝总皂苷(GPS)为目标,优化绞股蓝中GPS的纯化工艺,采用UPLC-QTOF-MS技术对GPS进行定性.“伴随标志物”紫外在线检测研究表明,上样终点为流出液吸光度为原药液的1/2时,洗脱终点为洗脱流出液吸光度基本稳定.UPLC-QTOF-MS鉴定出GPS提取物中的16个皂苷,其中新皂苷3个.该研究优选出的纯化工艺简单、检测方便、易于在线测定、实时记录,能较好的运用到大生产或生产的自动化中.质谱定性鉴定结果表明,基于“伴随标志物”紫外在线检测技术能很好地保留药材中的主要药效成分,为过程控制和质量溯源提供了分析工具.

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Key words

UPLC-QTOF-MS,adjoint marker,gynostemma pentaphyllum saponins (GPS),macroporous absorptive resin,online detecting,purification

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