Rapid Determination Of 10 Volatile N-Nitrosamines In Sour Meats By Modified Quechers And Gas Chromatography-Triple Quadrupole Mass Spectrometry

A rapid gas chromatography. triple quadrupole mass spectrometry (GC-MS/MS) method with modified QuEChERS method was developed for the determination of 10 volatile N- nitrosamines in sour meats. The samples were extracted with acetonitrile and eliminated the fat with n-hexane. The extracts in acetonitrile layer were purified by octadecyl silane (C18) and primary secondary amine (PSA) sorbents. The N. nitrosamines were determined on a DB-WAX column (30 m x0.. 25 mm x0.. 25 mu m) by GC-MS/MS in selected reaction monitoring (SRM) mode. The external standard method was used to quantify the N-nitrosamines. The correlation coefficients (r) of the standard calibration curves were greater than 0.. 99 in the range of 1-100 mu g/L. The average recoveries ranged from 79.. 8% to 115.. 3% with the relative standard devia. tions (RSDs) from 0.. 6% to 22.. 9% (n = 6). The limits of detection (LODs, S/N = 3) and limits of quantification (LOQs, S/N = 10) were 0.04-0.3 mu g/kg and 0.1-1 mu g/kg, respectively. The developed method is simple, rapid, sensitive and efficient for the screening of the N-nitrosamines in sour meats.