The exploration of endocytic mechanisms of PLA-PEG nanoparticles prepared by coaxialtri-capillary electrospray-template removal method.

•The nano-sized PLA-PEG particles were efficiently prepared by using coaxial tri-capillary electrospray-template removal method.•Caveolae-mediated endocytosis was one of endocytosis pathways in A549 cells for PLA-PEG nanoparticles.•Clathrin mediated endocytosis was not involved in the endocytosis process.•The endocytosed PLA-PEG nanoparticles enriched in the head of A549 cells.•A small amount of PLA-PEG nanoparticles was transported into lysosome after 24h incubation.