MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation.

Alan Van Goethem,Pieter Mestdagh,Tom Van Maerken,Jo Vandesompele

FUNCTIONAL GENOMICS: METHODS AND PROTOCOLS, 3RD EDITION（2017）

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摘要

miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.

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关键词

Normalization,RT-qPCR,Small RNA sequencing,miRNA,miRNA annotation

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