Optimized purification of a fusion protein by reversed-phase high performance liquid chromatography informed by the linear solvent strength model.

Isaac B Falconer,Colin T Mant,C James McKnight,Liliya Vugmeyster,Robert Hodges

Journal of Chromatography A（2017）

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摘要

•Optimized conditions for separating HP36 from the full fusion protein.•Reversed-phase HPLC using a shallow gradient.•Unexpected elution behavior at shallow gradients.•Elution behavior dependent on TFA concentration.

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关键词

Fusion proteins,Reversed-phase HPLC,Linear solvent strength model

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