麝香保心丸对异丙肾上腺素诱导的心肌细胞肥大及其Cx43蛋白表达的影响
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences(2017)
Abstract
目的:观察在麝香保心丸(Shexiang Baoxin Pills,SBP)的影响下异丙肾上腺素(isoprenaline,Iso)诱导的心肌细胞体积、样态及缝隙连接蛋白43(connexin 43,Cx43)表达的变化.方法:采用随机法将H9C2心肌细胞分为假手术组(sham组)、Iso组、Iso+SBP组,培养72 h后,在倒置相差显微镜下测定H9C2细胞表面积;采用聚氰基丙烯酸正丁酯(bicinchoninic acid,BCA)蛋白检测法测定细胞蛋白浓度;采用MTT比色法测定心肌细胞存活率;采用Western印迹检测Cx43蛋白的表达.结果:倒置相差显微镜下观察发现:与sham组比较,Iso组心肌细胞表面积和细胞蛋白浓度均增加(均P<0.05);而与Iso组相比,Iso+SBP组心肌细胞表面积减少,细胞蛋白浓度降低(均P<0.05);Western印迹显示:与sham组相比,Iso组H9C2细胞Cx43蛋白的表达水平明显下调(P<0.05);与Iso组相比,Iso+SBP组Cx43的表达水平明显上调(P<0.05).结论:麝香保心丸可改善Iso诱导的心肌细胞肥大,并能使Cx43蛋白表达下调.
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Key words
Shexiang Baoxin Pill,myocardial cell,connexin 43,myocardial hypertrophy,gap junction
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