Validation of an automated immune turbidimetric assay for serum gelsolin and its possible clinical utility in sepsis.
JOURNAL OF CLINICAL LABORATORY ANALYSIS(2018)
摘要
BackgroundStudies showing the potential predictive value of the actin-binding protein gelsolin, in critically ill patients are scarce. Moreover, even up to now a rapid automated measurement of gelsolin has still remained a challenge. Therefore, we developed and validated an automated serum gelsolin immune turbidimetric assay for possible clinical use. MethodsValidation of serum gelsolin assay was performed on a Cobas 8000/c502 analyzer (Roche) according to the second edition of Eurachem guidelines. Furthermore, we also studied the diagnostic value of serum gelsolin in sepsis when investigating sera of septic (n=25), systemic inflammatory response syndrome (SIRS; n=8) and control patients (n=14). We compared our previously published Western blot data with those of the new turbidimetric assay. ResultsThe sample volume was 7L and the assay time was 10minutes. The detection limit was 0.72mg/L, intra- and inter-assay imprecision remained in most cases less than 5% expressed as CV. Recovery was found to be 84.56%-93.52% and linearity study gave an appropriate correlation coefficient by linear regression analysis (r(2)=.998). Septic patients exhibited lower (P=.015) first-day serum gelsolin levels than SIRS patients, which confirmed our previous Western blot results. The determined cut-off point for serum gelsolin was 14.05mg/L (sensitivity: 75%; specificity: 60%) when investigating its diagnostic value in sepsis. ConclusionBased on the results, our immune turbidimetric measurement offers a rapid and accurate quantitation of gelsolin in human serum samples. Serum gelsolin seems a promising additional diagnostic marker of sepsis which has to be further investigated.
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关键词
automation,diagnostic marker,immune turbidimetry,sepsis,serum gelsolin
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