Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function

Makoto Kurachi, Junko Kurachi,Zeyu Chen,John Johnson,Omar Khan,Bertram Bengsch,Erietta Stelekati,John Attanasio,Laura M McLane,Michio Tomura,Satoshi Ueha,E John Wherry

NATURE PROTOCOLS（2017）

引用 38|浏览26

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摘要

This protocol describes an optimized procedure for retroviral (RV) transduction of mouse T cells by enriching RV-susceptible activated CD8 + T cells through Percoll density centrifugation, enhancing the frequency of RV-transduced cells ∼sixfold in vivo .

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关键词

Cytotoxic T cells,Flow cytometry,Genetic transduction,Immune cell isolation,Retrovirus,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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