Enhanced photobactericidal and targeting properties of a cationic porphyrin following attachment of Polymyxin B.
BIOCONJUGATE CHEMISTRY(2017)
Abstract
A novel compound consisting of a cationic porphyrin covalently attached to a derivative of polymyxin B has been synthesized and presents enhanced activity and targeting properties compared to the usual cationic porphyrins recognized as efficient photosensitizers in photodynamic antimicrobial chemotherapy (PACT). A synthesis pathway was established to preserve the bactericidal activity of the peptide. Accordingly, the N-terminal amino acid (L-2,4-diaminobutyric acid) of polymyxin B (PMB) was switched for a cysteine residue. Then, the resulting derivative of PMB was covalently bound to 5-(4-aminopheny1)-10,15,20-tri(4-Nmethylpyridy1)-21H,23H-porphyrin using a thiol maleimide "click" coupling. peptide-coupled photosensitizer has demonstrated an improved PACT efficiency compared to the cationic porphyrin alone This enhancement has been observed confocal. against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli in particular. Flow cytometry analyses and imaging microscopy demonstrated that the porphyrin peptide conjugate selectively adhered to the cell walls of either Grain positive or Gram-negative bacteria, thus justifying the damages induced by singlet oxygen production.
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Key words
cationic porphyrin,photobactericidal
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