Cloning and expression of the recombinant crustacean hyperglycemic hormone isoform B2 (rCHH-B2) and its effects on the metabolism and osmoregulation of the Pacific white shrimp Litopenaeus vannamei.
General and Comparative Endocrinology(2017)
摘要
•CHH-B2 from L. vannamei was expressed in yeast with a free C-terminus (rCHH-B2-Gly).•Dose-dependent effects of rCHH-B2-Gly to glucose and triglyceride were observed.•rCHH-B2-Gly modified the osmoregulatory capacity of hyper-osmotic shrimp.•rCHH-B2-amide enhanced the glucose and triglyceride compared to rCHH-B2-Gly.•The C-terminal region seems relevant for the activity of CHHs of L. vannamei.
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关键词
BMGY,BMMY,EDTA,MES,PAM,PBS,PCR,RP-HPLC,SDS-PAGE,TCA,TFA,TMB,YPD
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