Mucilaginibacter Craterilacus Sp Nov., Isolated From Sediment Soil Of A Crater Lake

A novel bacterial strain, designated N60A(T), was isolated from sediment soil of crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of N60A(T) were Gram-reaction-negative, oxidase-and catalase-positive, non-motile rods and formed transparent white colonies on ten-fold diluted R2A agar. N60A(T) contained summed feature 3 (C-16:1 omega 7c/C-16:omega 6c), iso-C-15:0 and iso-C-17:0 3-OH as the major fatty acids and MK-7 as the predominant isoprenoid quinone. It contained phosphatidylethanolamine as the predominant polar lipid. The DNA G+C content was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that N60A(T) formed a separate lineage in the genus Mucilaginibacter and that it was most closely related to Mucilaginibacter frigoritolerans FT22(T) (96.5% sequence similarity). Phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that N60A(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter craterilacus sp. nov. is proposed. The type strain is N60A(T) (= KCTC 52404(T) = NRRL B-65396(T)).