The cubicon method for concentrating membrane proteins in the cubic mesophase

Pikyee Ma,Dietmar Weichert,Luba A Aleksandrov,Timothy J Jensen,John R Riordan,Xiangyu Liu,Brian K Kobilka,Martin Caffrey

NATURE PROTOCOLS（2017）

引用 27|浏览47

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摘要

The lipid cubic phase ( in meso ) method is used for generating crystals and X-ray structures of integral membrane proteins. This protocol describes the cubicon method for concentrating membrane proteins in the cubic mesophase.

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关键词

Membrane biophysics,Membrane proteins,X-ray crystallography,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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