Ratiometric fluorescence detection of superoxide anion based on AuNPs-BSA@Tb/GMP nanoscale coordination polymers.
LUMINESCENCE(2018)
Abstract
A novel ratiometric fluorescence nanosensor for superoxide anion (O-2(center dot-)) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O-2(center dot-). The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O-2(center dot-) demonstrated high sensitivity and selectivity with a wide linear response range (14nM-10M) and a low detection limit (4.7nM).
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Key words
AuNPs-BSA@Tb/GMP,fluorescence nanosensor,nanoscale coordination polymers,ratiometric,superoxide anion
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