Dynamic transcription and expression patterns of KIF3A and KIF3B genes during spermiogenesis in the shrimp, Palaemon carincauda.
Animal Reproduction Science(2017)
Abstract
•The transcriptional level of KIF3A and KIF3B mRNA were analyzed.•The expression patterns of KIF3A/3B mRNA during spermiogenesis were analyzed.•Localization of KIF3A protein and organelles during spermiogenesis were analyzed.•KIF3A/3B will take part in acrosome formation and nuclear shaping.
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Key words
UTR,RACE,qPCR,ORF,IFT,KIFs,LCx,AFS,KAP3,PBS,OCT,DEPC,SSC,DIG,PFA,BSA,TEM,SD
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