A multi-tiered, in vivo, quantitative assay suite for environmental disruptors of thyroid hormone signaling.

•A Xenopus laevis induced metamorphosis assay suite detects thyroid hormone disruption.•Assays quantify thyroid-hormone-induced morphological changes, cellular proliferation, and gene expression.•Tetrabromobisphenol A inhibits a thyroid-hormone-response-element reporter gene in vivo.•Tetrabrominated diphenyl ether 47 toxicity is general, not thyroid hormone signaling specific in these assays.