Expression of fibronection fragments inE. coli

Summary Methods are described for high-efficiency expression of fibronectin fragments by using anE. coli protein expression system. Complementary DNA (cDNA) fragments cloned in a T7 promoter-based expression plasmid were expressed inE. coli strain BL21(DE3, pLysS). The resulting fibronectin fragments were purified by DEAE ion-exchange chromatography. This method can produce a large quantity of protein fragments for various studies including functional assays and biophysical measurements.