Spatial conservation studies of nucleobases in 10–23 DNAzyme by 2′-positioned isonucleotides and enantiomers for increased activity

2'-Positioned isonucleotides and enantiomers were used to evaluate the conservation of the spatial location of five adenines and two thymines in the catalytic core of 10-23 DNAzyme. The positive effect of isonucleotides at A15 and T8 along with inherent enzymatic resistance could be a tangible solution for the practical applications of 10-23 DNAzyme.