Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.
T-CELL DIFFERENTIATION: METHODS AND PROTOCOLS(2017)
摘要
MicroRNAs (miRNAs) are crucial components of the molecular networks regulating differentiation and responses of T lymphocytes in health and disease. It is therefore essential to rely on robust methods of qualitative and quantitative investigation of miRNA expression in T cell subsets, and during T cell activation and differentiation. Here, we focus on different methods for miRNA analysis, including Northern blots, quantitative RT-PCR, and next-generation sequencing, and we discuss advantages and disadvantages of each method. While we mainly focus on the study of miRNA expression in human T lymphocytes, these methods can also be applied to other species and/or cell types.
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关键词
T lymphocytes,CD4,CD8,MicroRNAs,RNA extraction,Northern blot,Next-generation sequencing,Quantitative RT-PCR
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