Determination of 3'-phosphoadenosine-5'-phosphosulfate in cells and Golgi fractions using hydrophilic interaction liquid chromatography-mass spectrometry.
Journal of Chromatography A（2016）
Abstract
•Selective detection of PAPS with LC–MS within 10min.•ZIC-pHILIC provided separation from potentially interfering ADP/ATP.•The multi-separation mechanisms of HILIC requires fine-tuning.•Ultracentrifugal filtration of cell samples for simple preparation.
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Key words
3′-Phosphoadenosine-5′-phosphosulfate,PAPS,HILIC,Sulfation,Golgi,pHILIC
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