TrackMate: an open and extensible platform for single-particle tracking.

•TrackMate is a software tool for automated, and semi-automated particle tracking.•TrackMate’s major development focus is on usability and extensibility.•TrackMate leverages Fiji to help provides its functionality and extensibility.•TrackMate is used for: C. elegans lineaging, NEMO assembly and clathrin dynamics.•Challenging imaging problems can robustly be analyzed using semi-automatic methods.