The Effect And Mechanism Of Action Of Metformin On Invitro Fadu Cell Proliferation
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH(2016)
摘要
Objective To investigate the effect and mechanism of action of metformin on proliferation of a human hypopharyngeal carcinoma cell line (FaDu).Methods FaDu cells were treated with metformin (25-125mmol/l). Cell proliferation was evaluated via CCK-8 assay. Real-time quantitative reverse transcription-polymerase chain reaction was used to evaluate microRNA (miR)-21-5p and PDCD4 (programmed cell death 4) expression. PDCD4 protein was quantified by Western blot.Results Metformin significantly inhibited FaDu cell proliferation in a dose- (25-100mmol/l) and time-dependent manner (12h-36h), significantly downregulated miR-21-5p, and upregulated PDCD4 mRNA and protein expression.Conclusions Metformin significantly inhibited FaDu cell proliferation, possibly via downregulation of miR-21-5p and upregulation of PDCD4.
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关键词
Hypopharyngeal carcinoma, metformin, FaDu cells, miR-21-5p, PDCD4, Real-time PCR, Western blot assay
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