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Single-use, electricity-free amplification device for detection of HIV-1.

Kelly A Curtis,Donna L Rudolph, Daphne Morrison,Dylan Guelig,Steven Diesburg,David McAdams,Robert A Burton,Paul LaBarre,Michele Owen

Journal of Virological Methods（2016）

Cited 27|Views5
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Abstract
•A rapid, single-use nucleic acid amplification device (NINA-SUD) was evaluated.•An exothermic chemical reaction provides the heat source for the NINA-SUD devices.•We demonstrated amplification from whole blood by HIV-1 RT-LAMP in the NINA-SUD.•Reproducibility was observed between NINA-SUD units and a thermal cycler.•Stability of the lyophilized HIV-1 RT-LAMP reagents was demonstrated.
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Key words
HIV-1,Diagnosis,Point-of-care,RNA,Nucleic acid amplification
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