A novel single cell method to identify the genetic composition at a single nuclear body
SCIENTIFIC REPORTS(2016)
摘要
Gene loci make specific associations with compartments of the nucleus (e.g. the nuclear envelope, nucleolus and transcription factories) and this association may determine or reflect a mechanism of genetic control. With current methods, it is not possible to identify sets of genes that converge to form a “gene hub” as there is a reliance on loci-specific probes, or immunoprecipitation of a particular protein from bulk cells. We introduce a method that will allow for the identification of loci contained within the vicinity of a single nuclear body in a single cell. For the first time, we demonstrate that the DNA sequences originating from a single sub-nuclear structure in a single cell targeted by two-photon irradiation can be determined and mapped to a particular locus. Its application to single PML nuclear bodies reveals ontologically related loci that frequently associate with each other and with PML bodies in a population of cells and a possible nuclear body targeting role for specific transcription factor binding sites.
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关键词
Biological techniques,Genetic techniques,Science,Humanities and Social Sciences,multidisciplinary
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