Ebola Virus Disease Diagnostics, Sierra Leone: Analysis Of Real-Time Reverse Transcription-Polymerase Chain Reaction Values For Clinical Blood And Oral Swab Specimens

Bobbie R Erickson,Tara K Sealy, Tim Flietstra,Laura Morgan,Brima Kargbo,Victor E Matt-Lebby,Aridth Gibbons,Ayan K Chakrabarti,James Graziano,Lance Presser,Mike Flint,Brian H Bird,Shelley Brown,John D Klena,Dianna M Blau,Aaron C Brault,Jessica A Belser,Johanna S Salzer,Amy J Schuh,Michael Lo,Marko Zivcec,Rachael A Priestley,Meredith Pyle,Christin Goodman,Scott Bearden,Brian R Amman,Alison Basile,Éric Bergeron,Michael D Bowen,Kimberly A Dodd,Molly M Freeman,Laura K McMullan,Christopher D Paddock,Brandy J Russell,Angela J Sanchez,Jonathan S Towner,David Wang,Galina E Zemtsova,Robyn A Stoddard,Maryann Turnsek,Lisa Wiggleton Guerrero,Shannon L Emery,Janae Stovall,Markus H Kainulainen,Jamie L Perniciaro,Slavica Mijatovic-Rustempasic, Gulchekhra Shakirova,Jörn Winter,Christopher Sexton,Feng Liu, Kimetha Slater,Raydel Anderson, Lauren Andersen,Cheng-Feng Chiang, Wen-Pin Tzeng,Samuel J Crowe,Matthew J Maenner,Christina F Spiropoulou,Stuart T Nichol,Ute Ströher

JOURNAL OF INFECTIOUS DISEASES（2016）

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摘要

During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.

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关键词

Ebola virus, West Africa, qRT-PCR, housekeeping gene, endogenous control

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