Kocuria Pelophila Sp Nov., An Actinobacterium Isolated From The Rhizosphere Of A Mangrove

A novel spherical actinobacterium, designated RS-2-3(T), was isolated from the rhizosphere of a mangrove growing on Rambut Island, Indonesia, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain RS-2-3(T) was related to the members of the genus Kocuria. The highest 16S rRNA gene sequence similarity value was observed with Kocuria marina KMM 3905(T) (97.0 %). The peptidoglycan type of strain RS-2-3(T) was found to be A3 alpha with an interpeptide bridge comprising L-Ala(4-5). The predominant menaquinone was MK-7(H-2) and the major fatty acids were anteiso-C-15 : 0 and iso-C-15 : 0. The DNA G+C content was 71.8 mol%. These characteristics were consistent with those of members of the genus Kocuria. Meanwhile, physiological and biochemical characteristics revealed that strain RS-2-3(T) differed from the species of the genus Kocuria with validly published names. Therefore, strain RS-2-3(T) represents a novel species of the genus Kocuria, for which the name Kocuria pelophila sp. nov. is proposed. The type strain is RS-2-3(T) (= NBRC 110990(T) = InaCC A704(T)).