165P: Long noncoding RNA LUCAT1 is associated with poor prognosis in human non-small cell lung cancer and affects cell proliferation via regulating p21 and p57 expression.
JOURNAL OF THORACIC ONCOLOGY(2016)
Abstract
were collected between July 2013 and November 2015. DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue, using the Cobas® DNA Sample Preparation Kit. For the mutational screening we used the Cobas® EGFR Mutation Test v2. performed on the Cobas® 4800 System. All mutations were detected at 5% sensitivity with the exception of the 2240_2257del18 exon 19 deletion mutation, detected at a sensitivity of >10%. Presence of more than 50% adequate tumor tissue was confirmed by a pathologist. Results: Histological evaluation found 380 (86.4%) cases with adenocarcinoma, 40 (10%) with squamous-cell carcinoma and 20 (4.6%) with NSCLCNST. From all 440 tested samples in 58 (13.2%) we found mutation in the EGFR gene. By 37 (63.8%) of them (15 male, 22 female) it was an activating mutation in EGFR gene and by 21 (36.2%) mutations were linked to insensitivity to TKIs. The prevalent type of activating EGFRmutation was deletion in exon 19 (in 24 cases – 64.9%), in 11 (29.7%) cases – L858 in exon 21, in 1 (2.7%) case – S768I in exon 20 and by one patient we found double heterozygosity – S768I in exon 20 and G719X in exon 18. Half of the patients were non-smokers. 97.3% of the tumor samples with activating mutations were adenocarcinomas, the rest with histology for squamous-cell carcinoma. 81.1% of the samples were from the primary tumor and the rest from the secondary metastasis. All patients with activating mutations were treated with first line TKIs according to guidelines. Conclusions: This study successfully provided insights into the prevalence and type of the EGFR gene mutation, which was detected in 13.2% of the studied Bulgarian population and corresponds to the publicly available data. Legal entity responsible for the study: Medical University Pleven, Department of Pathology Funding: Roche national program for patient support and EGFR testing Disclosure: All authors have declared no conflicts of interest.
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Key words
rna lucat1,lung cancer,non-small
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