Fractionation and analysis of lipopolysaccharide-derived oligosaccharides by zwitterionic-type hydrophilic interaction liquid chromatography coupled with electrospray ionisation mass spectrometry.
Carbohydrate Research(2016)
Abstract
•ZIC®-HILIC® is efficient for separation of LPS-derived core oligosaccharides.•ZIC®-HILIC®-ESI-MS enables separation and identification of core glycoforms.•The method provides separation based on O-acetyl, amine, Etn, and P and PP groups.
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Key words
Lipopolysaccharide,Core oligosaccharide,Hydrophilic interaction liquid chromatography,Zwitterionic stationary phase,Electrospray ionisation mass spectrometry
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