Adsorption of gastric lipase onto multicomponent model lipid monolayers with phase separation.

Claire Bourlieu,Gilles Paboeuf,Sophie Chever,Stéphane Pezennec,Jean-François Cavalier,Fanny Guyomarc'h,Amélie Deglaire,Saïd Bouhallab,Didier Dupont,Frédéric Carrière,Véronique Vié

Colloids and Surfaces B: Biointerfaces（2016）

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摘要

•Ellipsometry evidenced high amount of gastric lipase close to interface.•Gastric lipase adsorption impacts on lipid phase separation.•Gastric lipase partitions towards LE phase in monolayer with phase coexistence.•Gastric lipase adsorbs at variable levels of insertion suggesting variable interaction.•Lipase adsorption is mediated by hydrophobic and electrostatic interactions.

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关键词

AFM,DGL,DOPC,DPPC,DOPE,DOPS,HGL,IRS,GL,LE,LC,MPL,PC,PE,PI,PS,rDGL

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