Development of a cDNA‐AFLP derived CAPS marker co‐segregating with the powdery mildew resistance gene Mlg in barley

Powdery mildew is an important pathogen of barley in many barley-growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA-AFLP approach was carried out on near-isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by BLASTN, a CAPS marker has been developed, which co-segregated with Mlg in a population of 738 F-2-plants. Due to its co-dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker-based selection procedures.