Xanthine Oxidase Inhibitory Activity of Tetracera Indica

Preliminary screening study revealed that the methanolic extract of the stem of T. indica showed xanthine oxidase inhibitory activity in a concentration-dependent manner. EA fraction was selected to be further study due to its potential to inhibit xanthine oxidase enzyme with IC 50 value of 21.14 ∝g/ml which in lower than IC 50 value of MeOH extract, 42.02 ∝g/ml. Further separation and purification of EA fraction led to the isolation of two known compounds. Those compounds were identified by analysis of their spectroscopic data and comparisons with literature data to be betulinic acid and 5,7-dihydroxyl-8-methoxyflavone.