Gingival Crevicular Fluid Biomarkers in Patients with Gingivitis and Chronic Periodontitis

To quantify biomarkers of inflammation and tissue destruction in gingivo crevicular fluid to contribute to the diagnosis of the severity of the gingivo-periodontal disease. 115 adults with gingival-periodontal disease were included. Diagnosis was determined through clinical and radiographic criteria. Samples were obtained from six to eight sites per patient. Data were analyzed by an SPSS system using ANOVA One Way and Tukey tests. Volume raised in gingivitis, moderate and severe periodontitis Total proteins and hydroxyproline increased in moderate and severe periodontitis. Electrophoresis evidenced protein bands that increased with the severity of periodontitis. Some were similar to serum proteins and an additional band of less than 14 kDa was present in all the periodontitis samples. Collagenase bands evidenced differences between healthy and diseased subjects. Calcium determinations showed no statistically significant differences among groups. Levels of gingivo crevicular fluid biomarkers specific for inflammation and collagen degradation correlate with the clinical diagnosis in patients with gingivitis and chronic periodontitis.