Construction of cDNA Library of Hansenula anomala

To investigate the molecular mechanism of ester-producing of acetate yeast, a cDNA library was constructed based on Switching Mechanism At 5' end of the RNA Transcript (SMART) technique using Construction Kit. The result of the test showed that the primary constructed cDNA library had a cloning capacity of 3x10(6) and the length of the inserted fragment was between 1-3kb, the coverage of cDNA library was 74.07%. The quality of the library could meet the requirements.