Study of the Interaction between Three New Amantadine Schiff Bases and BSA by the Multi-Spectroscopic Method

The binding of three amantadine schiff bases (ASBs) to BSA was investigated by fluorescence spectroscopy, UV-Vis spectroscopy and circular dichroism (CD). The results showed that the fluorescence of BSA was quenched remarkably by these compounds via a combination of static and dynamic quenching Binding constant (K-a), number of binding sites (n), and the binding distance (r) between three ASBs and BSA were calculated in this study. Displacement experiments were performed to identify these compounds binding sites on BSA, and the results showed that site I was the main location for ASBs binding to BSA. Moreover, synchronous fluorescence spectra and CD data revealed that ASBs induced the conformational change of BSA.