Two microtubule-plus-end binding proteins LIS1-1 and LIS1-2, homologues of human LIS1 in Neurospora crassa

•It is the first time where two paralogues of human Lis1 are described.•We showed that the effect in nuclear migration is secondary to the effect on Mts.•Lack of LIS1-1 strongly affects vegetative growth, conidiation and branching.•LIS1-1 and LIS1-2 have high identity but LIS1-2 lacks of the coiled coil motif.•There is a different regulation of LIS1-1 and LIS1-2.