Bactericidal Action of Green Tea Extract and Damage to the Membrane of Escherichia coli O157 : H7
Biocontrol Science(2001)
Abstract
The antibacterial activity of green tea extract was investigated on 36 isolates of pathogens. The minimum inhibitory concentration of the extract against some isolates such as Escherichia coli O157: H7 was less than 250 μg/ml. The growth of the organism in culture broth was completely inhibited with 500 μg/ml of the extract. The organism was not detected by culturing on agar plates after 6 h incubation in phosphate buffered saline with 1, 000 μg/ml of the extract. It was observed by fluorescence microscopy and scanning electric microscopy that the cell membranes were damaged with 1, 000 μg/ml of the extract.
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Key words
green tea extract,escherichia coli
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