Hydrogen-Production of Hydrogen-Producing Bacteria at Different Temperatures in Batch Culture

Energy crisis is paid more attention to its significance around the world. Hydrogen is considered the most potential alternate energy source due to the character of non-pollution and zero emissions. This paper researched the variation of hydrogen-producing rate, pH value and the proportion under five temperatures of 25 degrees C, 30 degrees C, 35 degrees C, 40 degrees C, 45 degrees C through batch culture and the reasons of these appearance. And anaerobic hydrogen-producing bacteria's isolation and enrichment culture was accomplished by Hungater's anaerobic technique. The time of logarithmic phase was 24h, 16h, 12h, 20h and 28h and the stationary phase was 36h, 28h, 24h, 32h and 36h at 25 degrees C, 30 degrees C, 35 degrees C, 40 degrees C, 45 degrees C. When the pH declined to 4.2-4.4, the hydrogen-production rate and the proportion all reached optimal state. The maximum proportion of hydrogen-production and total gas-production was 70.41% at 35 degrees C. The optimal parameter was: the pH between 4.2-4.4 under the optimum temperature of 35 degrees C.