Esophageal smooth muscle cells dedifferentiate with loss of a-smooth muscle actin expression after 8 weeks of explant expansion in vitro culture: Implications on esophagus tissue engineering
European Surgery-acta Chirurgica Austriaca(2011)
摘要
Summary BACKGROUND: Esophagus tissue engineering using the hybrid-construct approach involves assembly of the esophageal components
to engineer the organ. This study investigated the dedifferentiation and loss in expression of esophageal smooth muscle after
explant expansion in culture. METHODS: Ovine esophagus smooth muscle cells (OESMC) were sourced from adult biopsies and expanded
using the explant culture technique. The explants were maintained under static in vitro tissue culture. Flow cytometry analysis was performed for α-smooth muscle actin (α-SMA) expression at intervals of 4 weeks
for up to 8 weeks. RESULTS: After 4 weeks 58.5% OESMC exhibited α-SMA which decreased to 28.5% after 6 weeks in culture. After
8 weeks in culture a mere 1.3% OESMC demonstrated α-SMA expression. CONCLUSIONS: OESMC proliferated using the explant technique
exhibit a total loss of α-SMA expression after 8 weeks of static in vitro culture. This information is crucial for tissue engineering of the esophagus using the hybrid-construct approach.
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关键词
tissue engineering,flow cytometry,tissue culture
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