Effects of Melatonin on Gene Expression of IVM/IVF Porcine Embryos
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(2005)
Abstract
The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR. Porcine embryos derived from in vitro maturation lin vitro fertilization were cultured in 5% CO2 and 20% O-2 at 37 degreesC in NCSU23 medium. Melatonin was added to medium at concentration of 1 nM, 5 nM, and 10 nM. When treated with 1 nM (39.0%) of melatonin, the developmental rate of embryos beyond the morula stage were higher than that of control group (31.0%) (p < 0.05). Number of inner cell mass and tropectoderm cell in control (23.0 +/- 0.5 and 17.3 +/- 0.8), 1 nM (23.6 +/- 0.6 and 19.0 +/- 0.5), and 5 nM (23.3 +/- 1.1 and 16.3 +/- 0.8) treated with melatonin were higher than in 10 nM (20.0 +/- 0.5 and 13.3 +/- 0.8) treated with melatonin (p < 0.05). To develop an mRNA phenotypic map for the expression of catalase, bax and caspase-3, single cell RT-PCR analysis were carried out in porcine IVM/IVF embryo. Catalase was detected in 0, 1 and 5 nM supplemented with melatonin, but bax and caspase-3 were detected in 10 nM treated with melatonin.
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Key words
porcine embryo,RT-PCR,catalase,bax,caspase-3,melatonin
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