Abstract 2491: Dynamics changes in antigen-autoantibody profiles involving glycolysis and spliceosome proteins precede a diagnosis of ER+ and triple negative breast cancer among post-menopausal women.

Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC We assessed the circulating autoantibody repertoire in breast cancer using genetically engineered mouse models and human plasmas collected at a pre-clinical time point and at the time of clinical diagnosis of breast cancer. We aimed to identify common pathways, networks and protein families associated with the humoral response to tumors and to elucidate the dynamic nature of tumor antigens and autoantibody interactions in ER+ and triple negative breast cancer. Lysate proteins from immortalized mouse cell lines from MMTV-neu and C3(1)-T mouse models and from the MCF7 and MDA-MB-231 human breast cancer cell lines were spotted onto nitrocellulose microarrays and hybridized with mouse and human plasma samples, respectively. Ig-based plasma immunoreactivity against glycolysis and spliceosome proteins was a predominant feature observed both in MMTV-neu tumor bearing mice and in pre-diagnostic ER+ human samples. A cytoskeletal signature was uniquely observed in C3(1)-T mice and in human patients with triple negative breast cancer. We provide evidence for dynamic changes in autoantibody reactivity and in antigen-antibody interactions with tumor development and progression. Citation Format: Jon Ladd, Melissa Johnson, Timothy Chao, Alice Chin, Sharon Pitteri, Jianning Mao, Lynn M. Amon, Martin McIntosh, Paul Lampe, Christopher Li, Ross Prentice, Nora Disis, Samir Hanash. Dynamics changes in antigen-autoantibody profiles involving glycolysis and spliceosome proteins precede a diagnosis of ER+ and triple negative breast cancer among post-menopausal women. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2491. doi:10.1158/1538-7445.AM2013-2491